Measurements of lactate concentration using lactate oxidase and an electrochemical oxygen sensor

Lactate oxidase was used in combination with an electrochemical dissolved oxygen sensor to measure L-lactate concentration in the physiological saline solution. The rate of oxygen consumption was found to have an excellent linear relationship with the lactate concentration on a log-log scale in the lactate concentration range of 0.3-25 mM. This detection does not require additional reagents and can be developed into a simple method of L-lactate determination. The effects of the temperature and pH of the solution on the reaction rate were investigated and discussed.