Nutrient-limited continuous culture in the phauxostat

Stable steady-state growth of Escherichia coli B limited by succinate, phosphate, or sulfate ion over the range of specific growth rates of 0.025-0.51 h(-1) was achieved using pH-controlled auxostasis in the phauxostat. The concentration of the growth-limiting substrate in the growth vessel could be varied at will in the region of the Monod half-maximal saturation constants by adjusting the concentration of that substrate in the reservior (at constant buffering capacity) or by varying the population density of the culture through changing the buffering capacity of the medium in the reservoir.