Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A two-generational reproductive study to assess the effects of Juglans regia on reproductive developments in the male and female rats.

Brazilian journal of biology = Revista brasleira de biologia·2023
Same author

Cardiometabolic health after first pregnancy: Associations with social determinants of health. A nuMoM2b-HHS study.

American heart journal plus : cardiology research and practice·2023
Same author

'One Health´ approach to end zoonotic TB.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2023
Same author

Prevalence of electronic cigarette usage among medical students in Saudi Arabia - A systematic review.

Nigerian journal of clinical practice·2022
Same author

A Pilot Study to Detect Agitation in People Living with Dementia Using Multi-Modal Sensors.

Journal of healthcare informatics research·2022
Same author

Determinants of Pre-Hospital Delay after Myocardial Infarction in Bangladesh: A Rural Center Experience.

Mymensingh medical journal : MMJ·2021

Related Experiment Video

Updated: Jul 4, 2026

Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry
11:37

Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry

Published on: November 29, 2013

Studies on chemically aggregated pepsin using glutaraldehyde.

S S Khan1, A M Siddiqi

  • 1Biochemistry Division, Department of Chemistry, Aligarh Muslim University, Aligarh 202001, UP, India.

Biotechnology and Bioengineering
|April 1, 1985
PubMed
Summary
This summary is machine-generated.

Immobilized porcine pepsin, created using glutaraldehyde, shows high yield and stability. This enzyme preparation is reusable and maintains activity across multiple assays, offering practical advantages.

More Related Videos

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
09:18

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Published on: April 17, 2017

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
11:14

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders

Published on: April 14, 2015

Related Experiment Videos

Last Updated: Jul 4, 2026

Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry
11:37

Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry

Published on: November 29, 2013

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
09:18

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

Published on: April 17, 2017

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders
11:14

Mass Spectrometric Approaches to Study Protein Structure and Interactions in Lyophilized Powders

Published on: April 14, 2015

Area of Science:

  • Biochemistry
  • Enzyme Technology
  • Protein Chemistry

Background:

  • Enzyme immobilization is crucial for industrial applications, enhancing stability and reusability.
  • Chemical aggregation using crosslinking agents like glutaraldehyde is a common method for enzyme immobilization.
  • Porcine pepsin is a key aspartic protease with various applications.

Purpose of the Study:

  • To chemically immobilize porcine pepsin using glutaraldehyde.
  • To characterize the kinetic and stability properties of the immobilized pepsin.
  • To evaluate the reusability and activity of the immobilized enzyme.

Main Methods:

  • Chemical immobilization of porcine pepsin via glutaraldehyde crosslinking.
  • Kinetic analysis using Michaelis-Menten parameters (K(m)).
  • Enzyme activity assays with hemoglobin and N-acetyl phenyl-alanyl-3, 5-diiodotyrosine (APDT) substrates.
  • Stability assessments against pH, temperature, urea, and guanidine hydrochloride.
  • Evaluation of enzyme reusability and storage stability.

Main Results:

  • Achieved 91% immobilization yield with Michaelis-Menten kinetics (K(m) = 5.3 x 10(-5) M).
  • Immobilized pepsin exhibited higher activation energy (90,613 cal/mol) compared to native pepsin (67,532 cal/mol).
  • Retained 67% and 79% activity with hemoglobin and APDT substrates, respectively.
  • Demonstrated significant stabilization against various denaturing agents and conditions.
  • Maintained 58% activity after 160 days of storage and nearly 100% activity over seven consecutive assays.

Conclusions:

  • Glutaraldehyde-mediated immobilization effectively stabilizes porcine pepsin, enhancing its operational robustness.
  • The immobilized enzyme exhibits excellent reusability and stability, making it suitable for continuous processes.
  • This method provides a practical approach for utilizing porcine pepsin in industrial settings.