Related Experiment Video
Updated: Jul 4, 2026

10:20
Mammalian Cell Encapsulation in Alginate Beads Using a Simple Stirred Vessel
Published on: June 29, 2017
Growth of animal cells on microbeads. I. In situ estimation of numbers
S J Miller1, M Henrotte, A O Miller
1Laboratory of Molecular Biochemistry, Faculty of Medicine, Mons State University, Avenue du Champ de Mars, 24-7000, Mons, Belgium.
Biotechnology and Bioengineering
|October 1, 1986
Abstract:
Evaluation of the number of anchorage-dependent cells growing on the surface of microbeads kept in suspension in a bioreactor is a tedious procedure fraught with many difficulties. Using the Coulter counter as a biomass probe, this article shows that the number of cells adhering to microbeads can be determined while the cells are still attached.
Related Concept Videos
Microbial Growth Measurement: Direct Methods
Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
Microbial Growth Measurement: Indirect Methods
Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...

