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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

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Related Experiment Video

Updated: Jul 4, 2026

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
11:33

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.

Published on: May 7, 2009

MicroRNAs and RNA interference in zebrafish development.

Gerrit Begemann1

  • 1LS Zoology and Evolutionary Biology, Department of Biology, University of Konstanz, Konstanz, Germany. gerrit.begemann@uni-konstanz.de

Zebrafish
|June 17, 2008
PubMed
Summary

MicroRNAs (miRNAs) are crucial regulators in zebrafish development, controlling cell migration, gene expression, and tissue regeneration. Further research into miRNA gene function is vital for understanding developmental genetics.

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Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides
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Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides

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Last Updated: Jul 4, 2026

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
11:33

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.

Published on: May 7, 2009

Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides
05:48

Micromanipulation of Gene Expression in the Adult Zebrafish Brain Using Cerebroventricular Microinjection of Morpholino Oligonucleotides

Published on: May 23, 2013

Area of Science:

  • Developmental Genetics
  • Molecular Biology
  • RNA Biology

Background:

  • Posttranscriptional gene regulation was historically understudied in developmental genetics.
  • The advent of RNA-mediated silencing mechanisms, particularly microRNAs (miRNAs), has revolutionized understanding of gene regulation.
  • Zebrafish models are increasingly used to study miRNA functions in development.

Purpose of the Study:

  • To review recent zebrafish studies highlighting the roles of miRNAs in various developmental processes.
  • To emphasize the significance of transcript inhibition mediated by miRNAs.
  • To explore the implications of miRNA involvement in genetic screens.

Main Methods:

  • Review of recent scientific literature focusing on zebrafish studies.
  • Analysis of experimental data demonstrating miRNA functions.
  • Discussion of techniques for manipulating miRNA activity.

Main Results:

  • miRNAs play critical roles in fine-tuning neural crest cell migration.
  • miRNAs regulate neural Hox gene expression during development.
  • miRNAs are involved in tissue regeneration after amputation and red blood cell maturation.
  • miRNA gene activity regulation is a subject of new discoveries.

Conclusions:

  • miRNAs are essential regulators across diverse developmental processes in zebrafish.
  • Specific phenotypes from single miRNA suppression suggest their targeted importance.
  • Future genetic screens should consider assays for miRNA genes and target sequences alongside protein-coding genes.