Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Preparation and Reactions of Thiols02:33

Preparation and Reactions of Thiols

Thiols are prepared using the hydrosulfide anion as a nucleophile in a nucleophilic substitution reaction with alkyl halides. For instance, bromobutane reacts with sodium hydrosulfide to give butanethiol.
Preparation and Reactions of Sulfides02:26

Preparation and Reactions of Sulfides

Sulfides are the sulfur analog of ethers, just as thiols are the sulfur analog of alcohol. Like ethers, sulfides also consist of two hydrocarbon groups bonded to the central sulfur atom. Depending upon the type of groups present, sulfides can be symmetrical or asymmetrical. Symmetrical sulfides can be prepared via an SN2 reaction between 2 equivalents of an alkyl halide and one equivalent of sodium sulfide.
2° Amines to N-Nitrosamines: Reaction with NaNO201:20

2° Amines to N-Nitrosamines: Reaction with NaNO2

Secondary amines react with nitrous acid to form N-nitrosamines, as depicted in Figure 1. Nitrous acid, a weak and unstable acid, is formed in situ from an aqueous solution of sodium nitrite and strong acids, such as hydrochloric acid or sulfuric acid, in cold conditions. In the presence of an acid, the nitrous acid gets protonated. The subsequent loss of water results in the formation of the electrophile known as nitrosonium ion.
Amines to Sulfonamides: The Hinsberg Test01:23

Amines to Sulfonamides: The Hinsberg Test

The Hinsberg test is a method to identify primary, secondary and tertiary amines, named after its pioneer, Oscar Hinsberg. Here, amines are treated with benzenesulfonyl chloride, also known as the Hinsberg reagent, in the presence of an excess of aqueous base, followed by acidification. Based on the nature of the amines, different changes are observed.
Generally, a primary amine reacts with the Hinsberg reagent to produce an N-substituted benzenesulfonamide. The electron-withdrawing sulfonyl...
Structure and Nomenclature of Thiols and Sulfides02:17

Structure and Nomenclature of Thiols and Sulfides

Thiols and sulfides are sulfur analogs of alcohols and ethers, respectively, where the sulfur atom takes the place of the oxygen atom. Thus, thiols are generally represented as RSH, where R is an alkyl substituent and —SH is the functional group. On the other hand, in sulfides, the central sulfur atom is bonded to two hydrocarbon groups on either side. Depending upon the type of group, sulfides can be either symmetrical or asymmetrical. Both thiols and sulfides display a bent geometry, similar...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of lifestyle factors on salivary nitrite and nitrate concentrations in overweight/obese individuals.

Archives of oral biology·2026
Same author

Latent transforming growth factor binding protein-2 (LTBP2), an IPF biomarker of clinical decline, promotes TGF-beta signaling and lung fibrosis in mice.

American journal of respiratory cell and molecular biology·2026
Same author

Anesthetic Implications and Physiologic Response to Transcatheter Pulmonary Flow Restrictor Placement in Neonates: A Single-Center Cohort Study.

Journal of cardiothoracic and vascular anesthesia·2026
Same author

Targeting the DNA damage repair protein RAD51 alters fibroblast metabolism and enhances apoptosis in pulmonary fibrosis.

bioRxiv : the preprint server for biology·2026
Same author

Viscous energy dissipation on hemolysis across various flow regimes using a high-shear device.

Medical engineering & physics·2026
Same author

Comparative Single-Cell Transcriptomics Uncovers Shared and Distinct Molecular Signatures in Cystic Fibrosis and Primary Ciliary Dyskinesia.

bioRxiv : the preprint server for biology·2025
Same journal

Clinical Europium fluorescent based lectin assays for mucin O-glycomics.

Methods in enzymology·2026
Same journal

A dual-color FRET assay for detection and quantitative analysis of O-glycopeptidases.

Methods in enzymology·2026
Same journal

Evolutionary genetic approaches to analyze mucins.

Methods in enzymology·2026
Same journal

Ex vivo imaging and enzymatic analysis of intestinal mucus.

Methods in enzymology·2026
Same journal

Glyco-TRAPP: A real-time glycocalyx permeability assay for assessing transmembrane mucin barrier function in live and fixed tissues.

Methods in enzymology·2026
Same journal

Quantitative imaging approaches to capture structural and functional dynamics of colonic mucus in health and disease in situ.

Methods in enzymology·2026
See all related articles

Related Experiment Video

Updated: Jul 4, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Novel method for measuring S-nitrosothiols using hydrogen sulfide.

Xinjun Teng1, T Scott Isbell, Jack H Crawford

  • 1Department of Pathology, University of Alabama at Birmingham, AL, USA.

Methods in Enzymology
|June 17, 2008
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method using hydrogen sulfide (H2S) to measure S-nitrosothiols (RSNOs). This technique offers a novel approach for studying nitric oxide (NO) biology and H2S

More Related Videos

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
08:25

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
03:55

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria

Published on: June 27, 2022

Related Experiment Videos

Last Updated: Jul 4, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
08:25

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
03:55

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria

Published on: June 27, 2022

Area of Science:

  • Biochemistry
  • Physiology
  • Analytical Chemistry

Background:

  • S-nitrosothiols (RSNOs) are crucial signaling molecules in nitric oxide (NO) biology.
  • Current methods for RSNO measurement often rely on reducing RSNOs to NO and detecting it via chemiluminescence.
  • Sensitive and specific measurement techniques are vital for understanding RSNO functions.

Purpose of the Study:

  • To introduce a novel reaction for measuring S-nitrosothiols (RSNOs) using hydrogen sulfide (H2S).
  • To demonstrate the adaptability of this H2S-based method for commercial nitric oxide (NO) analyzers.
  • To explore the potential of H2S as an experimental tool in RSNO research.

Main Methods:

  • Utilized a reaction chemistry where hydrogen sulfide (H2S) reduces RSNOs to nitric oxide (NO).
  • Employed ozone-based chemiluminescence in NO analyzers for sensitive NO detection under anaerobic conditions.
  • Investigated factors influencing H2S-based RSNO measurement.

Main Results:

  • Successfully adapted a novel H2S-based reaction for RSNO quantification.
  • Demonstrated the feasibility of using H2S for RSNO measurement with standard NO analyzers.
  • Identified key factors affecting the performance of H2S-based RSNO assays.

Conclusions:

  • Hydrogen sulfide (H2S) provides a viable alternative reagent for RSNO measurement.
  • The H2S-based method is readily adaptable to existing commercial NO detection systems.
  • H2S offers a promising new experimental tool for investigating RSNOs and their biological roles.