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Updated: Jul 4, 2026

In Vivo Calcium Imaging in C. elegans Body Wall Muscles
Published on: October 20, 2019
Expression and characterization of LTx2, a neurotoxin from Lasiodora sp. effecting on calcium channels
A A Dutra1, L O Sousa, R R Resende
1Laboratório de Biologia Celular e Molecular, Núcleo de Pesquisa em Ciências Biológicas, Departamento de Farmácia, Universidade Federal de Ouro Preto, Ouro Preto, MG 35400.000, Brazil.
Abstract:
Here, we described the expression and characterization of the recombinant toxin LTx2, which was previously isolated from the venomous cDNA library of a Brazilian spider, Lasiodora sp. (Mygalomorphae, Theraphosidae). The recombinant toxin found in the soluble and insoluble fractions was purified by reverse phase high-performance liquid chromatography (HPLC). Ca2+ imaging analysis revealed that the recombinant LTx2 acts on calcium channels of BC3H1 cells, blocking L-type calcium channels.
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