Related Experiment Video
Updated: Jul 4, 2026
Non-invasive Assessment of the Efficacy of New Therapeutics for Intestinal Pathologies Using Serial Endoscopic Imaging of Live Mice
Published on: March 10, 2015
Antioxidant potential by arabinoxylan rice bran, MGN-3/biobran, represents a mechanism for its oncostatic effect
Eman Noaman1, Nariman K Badr El-Din, Mona A Bibars
1Department of Radiation Biology, National Center for Radiation Research and Technology, Cairo, Egypt. emnoaman@yahoo.com
Abstract:
We have recently examined the oncolytic effect of arabinoxylan rice bran, MGN-3/biobran, against solid Ehrlich carcinoma (SEC)-bearing mice via immune-modulation and apoptosis [N.K. Badr El-din, E. Noaman, M. Ghoneum, In vivo tumor inhibitory effects of nutritional rice bran supplement MGN-3/biobran on Ehrlich carcinoma-bearing mice, Nutr. Cancer 60 (2) (2008) 235-244]. In the present study, we examined the antioxidant system as another possible mechanism through which MGN-3 exerts its oncostatic potential. Female albino mice were inoculated intramuscularly in the right thigh with Ehrlich ascites carcinoma (EAC) cells. MGN-3 (25 mg/kg body weight) was injected intraperitoneally (i.p.) six times a week for 25 days into mice at either day 4 or day 11 post-EAC cell inoculation. Tumor growth, lipid peroxidation (LPx), glutathione (GSH) contents, the activity of the antioxidant scavenger enzymes, and alterations in gene expression were examined. MGN-3 efficiently suppressed the growth of tumors, which was associated with normalization of the LPx levels and augmentation of GSH contents. MGN-3 enhanced the activity of the endogenous antioxidant scavenging enzymes -- superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione-S-transferase (GST) -- in blood, liver, and tumor tissue. Similarly it up-regulated the expression of GPx, SOD1 and CAT mRNA in the liver. The effect of MGN-3 was more pronounced when treated early, at day 4 of tumor cell inoculation, as compared to later treatment at 11 days. In conclusion, MGN-3-induced oncostatic activity by modulating lipid peroxidation, augmenting the antioxidant defense system and protecting against oxidative stress.
Insights
Arabinoxylan rice bran (MGN-3/biobran) suppresses tumor growth by enhancing the antioxidant system and reducing oxidative stress. Early treatment showed more pronounced effects on Ehrlich carcinoma in mice.
Area of Science:
- Biochemistry
- Oncology
- Immunology
Background:
- Arabinoxylan rice bran (MGN-3/biobran) has demonstrated oncolytic effects through immune modulation and apoptosis.
- The antioxidant system's role in MGN-3/biobran's oncostatic potential requires further investigation.
Purpose of the Study:
- To investigate the antioxidant system as a mechanism for the oncostatic effects of MGN-3/biobran.
- To evaluate the impact of MGN-3/biobran on lipid peroxidation, glutathione levels, antioxidant enzyme activity, and gene expression in a mouse model of Ehrlich carcinoma.
Main Methods:
- Ehrlich ascites carcinoma (EAC) cells were inoculated into female albino mice.
- MGN-3/biobran (25 mg/kg) was administered intraperitoneally at day 4 or day 11 post-inoculation for 25 days.
- Tumor growth, lipid peroxidation (LPx), glutathione (GSH) content, antioxidant enzyme activity (SOD, GPx, CAT, GST), and gene expression (GPx, SOD1, CAT mRNA) were assessed.
Main Results:
- MGN-3/biobran significantly suppressed tumor growth.
- Treatment normalized LPx levels and increased GSH content.
- MGN-3/biobran enhanced the activity of endogenous antioxidant enzymes in blood, liver, and tumor tissue.
- Gene expression of GPx, SOD1, and CAT mRNA was upregulated in the liver.
- Early treatment (day 4) yielded more significant results than later treatment (day 11).
Conclusions:
- MGN-3/biobran exhibits oncostatic activity by modulating lipid peroxidation and enhancing the antioxidant defense system.
- The supplement protects against oxidative stress, contributing to its tumor-inhibitory effects.
- Early intervention with MGN-3/biobran is more effective in managing Ehrlich carcinoma in this model.