Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Ni-rGO Sensor Combined with Human Olfactory Receptor-Embedded Nanodiscs for Detecting Gas-Phase DMMP as a Simulant of Nerve Agents.

ACS sensors·2023

Same author

Enhanced osteogenic differentiation of human mesenchymal stem cells using size-controlled graphene oxide flakes.

Biomaterials advances·2022

Same author

Facile synthesis of size-controlled Fe<sub>2</sub>O<sub>3</sub> nanoparticle-decorated carbon nanotubes for highly sensitive H<sub>2</sub>S detection.

RSC advances·2022

Same author

Optorheological Characteristics of Photosynthetic Bacterium Suspension.

Langmuir : the ACS journal of surfaces and colloids·2021

Same author

Comparative Studies on Crystallinity, Thermal and Mechanical Properties of Polyketone Grown on Plasma Treated CVD Graphene.

Polymers·2021

Same author

Ruthenium Decorated Polypyrrole Nanoparticles for Highly Sensitive Hydrogen Gas Sensors Using Component Ratio and Protonation Control.

Polymers·2020

Search research articles

Related Experiment Video

Updated: Jul 4, 2026

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

Published on: January 10, 2017

Label-free target DNA recognition using oligonucleotide-functionalized polypyrrole nanotubes.

Sungrok Ko¹, Jyongsik Jang

¹Hyperstructured Organic Materials Research Center and School of Chemical and Biological Engineering, Seoul National University, San 56-1 Shinlimdong, Gwanakgu, Seoul 151-742, Republic of Korea.

Ultramicroscopy

|June 17, 2008

Summary

Researchers developed novel polypyrrole nanotubes for DNA detection. These functionalized nanotubes enable sensitive detection of DNA hybridization, offering a promising platform for biosensing applications.

More Related Videos

A Polyaniline-based Sensor of Nucleic Acids

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

Published on: October 25, 2017

Related Experiment Videos

Last Updated: Jul 4, 2026

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

Engineering Molecular Recognition with Bio-mimetic Polymers on Single Walled Carbon Nanotubes

Published on: January 10, 2017

A Polyaniline-based Sensor of Nucleic Acids

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

DNA Nanotubes as a Versatile Tool to Study Semiflexible Polymers

Published on: October 25, 2017

Area of Science:

Materials Science
Nanotechnology
Biotechnology

Background:

Conjugated polymers are excellent for oligonucleotide immobilization and DNA detection.
Carboxylated conducting polymers offer versatile immobilization for biomolecules.

Purpose of the Study:

To fabricate carboxylic acid-functionalized polypyrrole nanotubes (CPPy NTs).
To utilize CPPy NTs for sensitive detection of DNA duplex formation.

Main Methods:

Fabrication of CPPy NTs using oxidant-impregnated template synthesis.
Immobilization of amino-terminal oligonucleotides onto CPPy NTs.
Measurement of DNA hybridization using conductance changes on gold leads.

Main Results:

High sensitivity (ΔR/R(0)=1.7) in detecting low concentrations (1 nmol) of target DNA.
Distinguished target DNA concentrations with a narrow difference of 2 nmol.
Confirmed DNA immobilization via confocal laser scanning microscopy of FITC-tagged DNA.

Conclusions:

CPPy NTs are effective platforms for oligonucleotide immobilization and DNA biosensing.
The developed method allows for sensitive and specific detection of DNA hybridization.
This approach holds potential for advanced diagnostic tools and molecular detection systems.