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Cell Motility through Blebbing

Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
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Articles linked to this work by shared authors, journal, and citation graph.

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Blimp-1's maiden flight.

Journal of immunology (Baltimore, Md. : 1950)·2010
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Blimp1-mediated repression of negative regulators is required for osteoclast differentiation.

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Related Experiment Video

Updated: Jul 4, 2026

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
09:32

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

Activation-dependent induction of Blimp-1.

Kathryn Calame1

  • 1Department of Microbiology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA. KLC1@columbia.edu

Current Opinion in Immunology
|June 17, 2008
PubMed
Summary

B lymphocyte induced maturation protein-1 (Blimp-1) mRNA has distinct roles in T and B cells. While B cells use multiple repressors, T cells primarily use Bcl-6, with different activators inducing Blimp-1 in each lineage.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • B lymphocyte induced maturation protein-1 (Blimp-1) is critical for lymphocyte function.
  • Blimp-1 plays distinct roles in T and B cell lineages following antigen-dependent activation.
  • Understanding Blimp-1 regulation is key to deciphering immune responses.

Purpose of the Study:

  • To elucidate the differential regulation of Blimp-1 mRNA induction in T and B lymphocytes.
  • To identify repressors and activators involved in Blimp-1 expression in distinct immune cell types.
  • To compare the mechanisms controlling Blimp-1 function across lymphocyte lineages.

Main Methods:

  • Analysis of Blimp-1 mRNA induction upon lymphocyte activation.
  • Investigation of regulatory mechanisms, including repressors and activators.

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Optogenetic Signaling Activation in Zebrafish Embryos
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Optogenetic Signaling Activation in Zebrafish Embryos

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Last Updated: Jul 4, 2026

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
09:32

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells
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Optogenetic Signaling Activation in Zebrafish Embryos

Published on: October 27, 2023

  • Comparative study of T cell and B cell signaling pathways.

    • Main Results:

    • B cells employ at least four mechanisms to repress Blimp-1, requiring relief before induction.
    • T cells utilize a single known repressor, Bcl-6.
    • Specific cytokines (IL-21, IL-10, IL-6, IL-2) and transcription factors (STAT3, AP-1, NF-kappaB, IRF4) differentially activate Blimp-1 in T and B cells, alongside toll-like receptor (TLR) signals in B cells.

    Conclusions:

    • Blimp-1 regulation is complex and lineage-specific, involving distinct sets of repressors and activators.
    • Differential control of Blimp-1 contributes to the specialized functions of T and B lymphocytes.
    • Identifying these regulatory pathways offers insights into immune cell differentiation and function.