Fabrication of reusable enzyme brushes for clinical analyses: Lactate dehydrogenase enzyme brush

A simple procedure for the entrapment of enzymes in nylon-reinforced cellulose triacetate fibers and the fabrication of reusable enzyme brushes using such fiber bristles for the analytical determination of unknown samples have been described. As an example, enzyme brushes of lactate dehydrogenase were fabricated and were repeatedly employed for the determination of pyruvic acid in clinical samples in place of soluble enzymes. The above brush method compared well with that of wet enzymic analysis of pyruvate in clinical samples in accuracy, sensitivity, and reproducibility. The device also proved to be easy to handle, use, and reprocess for repeated analyses.