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Application of immobilized heparins for isolation of human antithrombin III

G Mitra1, E Hall, I Mitra

  • 1Biological Research and Development, Cutter Laboratories, Fourth and Parker Streets, Berkeley, California 94710, USA.

Biotechnology and Bioengineering
|February 1, 1986
PubMed

Immobilized heparins were prepared by six different methods, and these were utilized for affinity purification of human antithrombin III (AT-III). Affinity support capacities (mg AT-III/g support) were strongly influenced by immobilized active heparin concentrations. In the temperature range 5-37 degrees C, colder temperatures favored affinity adsorption of AT-III as well as nonspecific interactions of all proteins. For representative human-plasma-derived feed solutions the selectivity for AT-III on the affinity support was dependent on relative concentrations of non-AT-III proteins as well as the specific mode of adsorption and elution (batch/continuous).

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