Related Experiment Video
Updated: Jul 4, 2026

08:13
A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Bubble coalescence in air-sparged bioreactors
1Department of Chemical Engineering, University of California, Berkeley, California 94720, USA.
Biotechnology and Bioengineering
|April 1, 1986
Summary
Coalescence frequency in bubble columns was measured using tracer gases. Increasing gas-sparge rates boosted frequency, while NaCl concentration decreased it, impacting bubble size.
Area of Science:
- Chemical Engineering
- Fluid Dynamics
- Multiphase Flow Systems
Background:
- Bubble columns are widely used in chemical processes for gas-liquid reactions and separations.
- Understanding bubble coalescence is crucial for optimizing mass transfer and reactor performance.
- Previous studies have explored factors affecting bubble dynamics, but comprehensive analysis of coalescence frequency under varying conditions is ongoing.
Purpose of the Study:
- To quantify the coalescence frequency in an air-sparged bubble column.
- To investigate the influence of gas-sparge rate and salt (NaCl) concentration on bubble coalescence.
- To correlate changes in coalescence frequency with bubble size distributions.
Main Methods:
- Coalescence frequency was determined by tracking the mixing rate of insoluble tracer gases within the dispersed gas phase.
- Experiments were conducted in an air-sparged bubble column.
- Bubble size distributions were measured using established techniques.
Main Results:
- Coalescence frequency increased with gas-sparge rate, correlating with gas-phase hold-up.
- NaCl concentration significantly reduced coalescence frequency, reaching approximately half that in pure water.
- Bubble size distribution showed notable changes with NaCl concentration, correlating with observed variations in coalescence frequency.
Conclusions:
- Gas-sparge rate and salt concentration are critical parameters influencing bubble coalescence in air-sparged columns.
- NaCl addition suppresses bubble coalescence, leading to altered bubble size distributions.
- The findings provide valuable insights for designing and operating bubble column reactors for enhanced efficiency.
Related Concept Videos
Bioreactor Controls-II
In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...
Bioreactor Design and Operational System
Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
Bioreactor Controls-I
Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...
Designing Growth Media for Bioreactors
Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...
Coagulation
Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...
Bioremediation
Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.

