Related Experiment Video

Updated: Jul 4, 2026

Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice
07:17

Phenotypic and Functional Analysis of Activated Regulatory T Cells Isolated from Chronic Lymphocytic Choriomeningitis Virus-infected Mice

Published on: June 22, 2016

Stimulatory and suppressive signal transduction regulates vasoactive intestinal peptide receptor-1 (VPAC-1) in

Emilie E Vomhof-DeKrey1, Glenn Paul Dorsam1

  • 1Department of Chemistry and Molecular Biology, The Center for Protease Research, North Dakota State University, Room 320 IACC Building, 1320 Albrecht Boulevard, Fargo, ND 58105, USA.

Brain, Behavior, and Immunity
|June 17, 2008
PubMed

Insights

This study reveals that JNK kinases suppress Vasoactive intestinal peptide receptor-1 (VPAC-1) mRNA levels in T cells upon activation. Other signaling pathways, like PKC and ERK, stimulate VPAC-1 in the absence of T cell signaling.

Area of Science:

  • Immunology
  • Cell Signaling

Background:

  • Vasoactive intestinal peptide receptor-1 (VPAC-1) is a G-protein coupled receptor expressed on T cells.
  • VPAC-1 is downregulated upon T cell activation, but the underlying signaling pathways remain unclear.

Purpose of the Study:

  • To investigate the signaling pathways regulating VPAC-1 expression in murine CD4 T cells.
  • To identify molecules involved in the downregulation of VPAC-1 during T cell activation.

Main Methods:

  • Murine splenic CD4 T cells were treated with pharmacological inhibitors.
  • Cells were stimulated with anti-CD3 antibody for 24 hours.
  • VPAC-1 mRNA levels were quantified using qPCR.

Main Results:

  • JNK kinases, downstream of Zap70, suppress VPAC-1 mRNA levels following anti-CD3 stimulation.
  • Inhibitors of PKC, ERK, p38, Zap70, and Rac1 indicated a stimulatory role in VPAC-1 regulation without T cell signaling.

Conclusions:

  • JNK signaling pathway plays a suppressive role in VPAC-1 regulation post-T cell activation.
  • Understanding VPAC-1 regulation offers insights into its role in inflammatory and autoimmune diseases.

More Related Videos

In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function09:26

In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function

Published on: October 15, 2013

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice08:37

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

Published on: April 21, 2015

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
GPCRs Regulate Adenylyl Cylase Activity01:09

GPCRs Regulate Adenylyl Cylase Activity

Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of cells.
Two...