A two-generation reproduction study in rats receiving diets containing hexamethylenediamine

Rats received diets containing average daily doses of 0, 50, 150, and 500 mg/kg/day of hexamethylenediamine over two generations. Although no treatment-related mortality was observed in any of the groups, the weight gain of adults and pups was slightly reduced in the high dose group. While the litter size was also slightly reduced at birth in the high dose group, there was no adverse effect on survival during lactation in any of the treated groups. Thus, the dietary administration of up to 150 mg/kg/day of hexamethylenediamine over two generations did not adversely affect reproduction or fertility in rats.