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Cell Population Analyses During Skin Carcinogenesis
06:53

Cell Population Analyses During Skin Carcinogenesis

Published on: August 21, 2013

Basal and UV-induced MMP-1 expression are inhibited by p53 in human dermal fibroblasts

Sangmin Kim1, Youngae Lee, Dong Hun Lee

  • 1Department of Dermatology, Seoul National University College of Medicine, Seoul National University Hospital, Seoul National University, Seoul, Korea.

Experimental Dermatology
|June 19, 2008
PubMed

Insights

The tumor suppressor p53 inhibits matrix metalloproteinase-1 (MMP-1) expression in human skin fibroblasts. This finding reveals a new role for p53 in regulating MMP-1, a key enzyme in skin aging and damage.

Area of Science:

  • Dermatology
  • Molecular Biology
  • Biochemistry

Background:

  • Ultraviolet (UV) radiation induces matrix metalloproteinase-1 (MMP-1) and p53 protein in human skin.
  • The precise role of p53 in regulating MMP-1 expression in human skin remains unclear.

Purpose of the Study:

  • To investigate the effect of p53 on basal and UV-induced MMP-1 expression in human dermal fibroblasts.
  • To elucidate the regulatory mechanism of p53 on MMP-1.

Main Methods:

  • Adenoviral p53 (Ad-p53) gene delivery into human dermal fibroblasts.
  • Treatment with p53 inhibitor (pifithrin-alpha) and p53 activator (etoposide).
  • Analysis of MMP-1 expression in normal and p53-mutated HaCaT cells.

Main Results:

  • Ad-p53 infection inhibited both basal and UV-induced MMP-1 expression.
  • Pifithrin-alpha augmented UV-induced MMP-1 expression, while etoposide decreased it.
  • Etoposide and pifithrin-alpha did not affect MMP-1 in p53-mutated HaCaT cells.

Conclusions:

  • p53 acts as an inhibitor of basal and UV-induced MMP-1 expression in human dermal fibroblasts.
  • These findings highlight a novel inhibitory role for p53 in MMP-1 regulation within the skin.

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