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Natural anti-endothelial cell antibodies
Amélie Servettaz1, Philippe Guilpain, Nicolas Tamas
1Université Paris Descartes, Faculté de Médecine, UPRES-EA 4058, Paris, France.
Autoimmunity Reviews
|June 19, 2008
Summary
Natural anti-endothelial cell antibodies (AECA) found in healthy individuals target common cellular proteins. These natural AECA may have anti-inflammatory roles and help control endothelial cell activation.
Area of Science:
- Immunology
- Cell Biology
- Proteomics
Background:
- Anti-endothelial cell antibodies (AECA) are found in autoimmune diseases but also in healthy individuals.
- The targets and functions of AECA in healthy individuals are not well understood.
- Most research focuses on pathological roles of AECA, neglecting their presence in healthy populations.
Purpose of the Study:
- To identify the specific protein targets of natural AECA in healthy individuals.
- To explore the potential physiological roles of these natural AECA.
Main Methods:
- Proteome-based technologies were employed to identify antigens recognized by natural AECA.
- Analysis of IgG AECA in sera from healthy individuals against endothelial cell protein extracts.
Main Results:
- Healthy individuals possess IgG AECA that recognize a specific set of endothelial cell proteins.
- Identified targets include ubiquitous cytoskeletal proteins (beta actin, vimentin, alpha tubulin) and glycolytic enzymes (glyceraldehyde-3-phosphate-deshydrogenase, alpha-enolase).
- These proteins are crucial for fundamental cellular functions.
Conclusions:
- Natural AECA in healthy individuals target highly conserved, physiologically important proteins.
- These natural AECA may possess anti-inflammatory and anti-thrombotic functions.
- They could play a role in regulating endothelial cell activation.
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