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Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
Published on: September 2, 2019
Diversity and stability study on rice mutants induced in space environment
Wei-Hong Lu1, Xin-Zhu Wang, Qi Zheng
1Department of Astronautic Engineering, Harbin Institute of Technology, Harbin 150001, China.
Genomics, Proteomics & Bioinformatics
|June 19, 2008
Summary
Spaceflight induced stable molecular changes in rice mutants. Specific seed proteins, albumin, globulin, and prolamine, can serve as biomarkers for identifying spaceflight-induced rice mutants.
Area of Science:
- Plant Molecular Biology
- Space Biology
- Proteomics
Background:
- Spaceflight can induce genetic and molecular alterations in plants.
- Understanding these changes is crucial for long-term space exploration and agriculture.
- Rice mutants from spaceflight offer a model to study induced molecular variations.
Purpose of the Study:
- To investigate molecular-level protein changes in rice mutants after spaceflight exposure.
- To compare protein profiles of spaceflight-induced mutants with ground controls.
- To identify stable molecular markers for spaceflight-induced rice mutants.
Main Methods:
- Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE)
- Reverse-phase liquid chromatography (RPLC)
- Ultra-violet spectrometry
- Mass spectrometry and database searching
Main Results:
- Low-abundance leaf proteins at peak tillering were more affected in mutants.
- Albumin, globulin, and prolamine in mutant seeds showed significant changes compared to controls.
- These protein changes were stably inherited across the 8th and 9th generations.
- Two proteins, SSP9111 and SSP6302, showed high-intensity expression and correlation with the photosystem.
Conclusions:
- Specific seed proteins (albumin, globulin, prolamine) are reliable biomarkers for identifying spaceflight-induced rice mutants.
- The identified protein changes are stably inherited, confirming their utility.
- SSP9111 and SSP6302, linked to the photosystem, are key proteins affected by spaceflight in rice.
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