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Published on: June 24, 2018
An evaluation of bacterial contamination of barriers used in periapical tissue regeneration: Part 1--Bacterial
Priya Sharma1, André K Mickel, Sami Chogle
1Department of Endodontics, Prosthodontics, and Operative Dentistry, Baltimore College of Dentistry, University of Maryland, Baltimore, MD 21201, USA. PChand@umaryland.edu
Objective:
To compare the adherence of Prevotella melaninogenica and Enterococcus faecalis to 3 guided tissue regeneration membranes: Atrisorb, Lambone, and OsseoQuest. It was hypothesized that OsseoQuest would show increased bacterial adherence compared to Lambone and Atrisorb.
Method And Materials:
The barriers were suspended in trypticase soy broth containing an inoculum of either P melaninogenica or E faecalis. The samples were incubated under appropriate conditions for 6, 24, and 48 hours. Following incubation, each membrane was mixed in fresh media in a vortex machine to dislodge adherent bacteria. The vortexed media was quantitatively assessed using serial dilutions for viable cell count.
Results:
E faecalis exhibited higher adherence compared to P melaninogenica with time. Of the membranes tested, Lambone displayed the least bacterial adherence.
Conclusion:
An analysis of the results indicated that bacterial adherence was time-dependent for all membranes. Membrane structure, chemical configuration, hydrophobicity, and bacterial cell surface structure were suggested as factors contributing to variance in bacterial adherence.
