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Related Experiment Video

Updated: Jul 4, 2026

Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS

Standards for Quantitative Metalloproteomic Analysis Using Size Exclusion ICP-MS

Published on: April 13, 2016

Metallomics and metalloproteomics.

W Shi¹, M R Chance

¹Center for Synchrotron Biosciences, Case Center for Proteomics, Case Western Reserve University, 10900 Euclid Ave, BRB 113, Cleveland, Ohio, 44106, USA.

Cellular and Molecular Life Sciences : CMLS

|June 19, 2008

Summary

Metallomics and metalloproteomics investigate essential trace metals in proteins. These fields use techniques like mass spectrometry and spectroscopy to understand metal-protein interactions and functions.

Area of Science:

Biochemistry
Analytical Chemistry
Bioinformatics

Background:

Metallomics and metalloproteomics are crucial for understanding the essential roles of trace metals in protein function.
These fields examine the uptake, transport, and storage of metals within biological systems.
Metalloproteins are vital for numerous cellular processes.

Purpose of the Study:

To discuss methodologies used in metallomics and metalloproteomics.
To highlight techniques for identifying and quantifying metalloproteins.
To explore the functional analysis of metal-protein complexes.

Main Methods:

Inductively coupled plasma mass spectrometry (ICP-MS) for metal identification.
Electrospray ionization mass spectrometry (ESI-MS) for protein structure and function.

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Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides

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X-ray absorption spectroscopy (XAS) and X-ray fluorescence spectroscopy (XRF) for direct metal analysis.

Bioinformatics for identifying metalloproteins in genomic data.

Main Results:

ICP-MS and ESI-MS are widely used for metal-protein analysis.
Synchrotron-based XAS provides direct insights into metal binding and distribution.
Bioinformatic tools aid in the discovery of novel metalloproteins from sequence data.

Conclusions:

A combination of analytical techniques and bioinformatics is essential for metallomics and metalloproteomics research.
These fields are advancing our understanding of metal-protein interactions and their biological significance.
Continued development of methodologies will enhance the study of metalloproteins.