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Plasmid Stability Analysis with Open-Source Droplet Microfluidics
Published on: December 27, 2024
Effects of dissolved oxygen shock on the stability of recombinant Escherichia coli containing plasmid pKN401
D J Hopkins1, M J Betenbaugh, P Dhurjati
1Department of Chemical Engineering, University of Delaware, Newark, Delaware 19716, USA.
Abstract:
The effect of dissolved oxygen shock on the stability of recombinant Escherichia coli cells containing plasmid pKN401 was investigated. The recombinant cells were stable in control batch experiments in media with and without ampicillin. However, these recombinant cells were highly unstable under conditions where a dissolved oxygen shock was induced. The results have implications for design of aerated reactors for recombinant cells.
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