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Related Concept Videos

Immunodeficiency Diseases01:25

Immunodeficiency Diseases

Immunodeficiency disorders are conditions in which the immune system's ability to fight infectious disease and cancer is compromised or entirely absent. The immune system comprises a complex network of cells, tissues, and organs that work together to protect the body from potentially harmful invaders. When this system is deficient or not functioning properly, it leaves the body susceptible to infections, diseases, or other complications.
There are three main causes of immunodeficiency disorders...
Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

Overview
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...

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Related Experiment Video

Updated: Jul 4, 2026

Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique
09:37

Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique

Published on: September 23, 2022

Flowcytometric phenotyping of common variable immunodeficiency.

Klaus Warnatz1, Michael Schlesier

  • 1Division of Rheumatology and Clinical Immunology, University Medical Center Freiburg, Freiburg, Germany. klaus.warnatz@uniklinik-freiburg.de

Cytometry. Part B, Clinical Cytometry
|June 19, 2008
PubMed
Summary

Flowcytometry is a key tool for classifying common variable immunodeficiency (CVID) by analyzing T and B cell subpopulations. This method helps identify genetic defects, aiding in diagnosis and patient stratification.

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Area of Science:

  • Immunology
  • Clinical diagnostics

Background:

  • Common variable immunodeficiency (CVID) is a heterogeneous group of antibody deficiency disorders.
  • Classifying CVID requires combining clinical and immunological parameters.
  • Flowcytometric analysis of lymphocyte subpopulations is crucial for CVID classification.

Purpose of the Study:

  • To assess the utility of flowcytometry in classifying CVID patients.
  • To evaluate flowcytometric screening for genetic defects associated with CVID.

Main Methods:

  • Multicolor flowcytometric analysis of circulating T and B lymphocytes.
  • Analysis of inducible costimulator on T cells, CD19, and BAFF-R on B cells.

Main Results:

  • Flowcytometry effectively identifies distinct patient groups within the CVID population.
  • Flowcytometric analysis serves as a valid screening method for three out of four known CVID genetic defects.
  • TACI deficiency is not sufficiently detectable by current flowcytometric methods.

Conclusions:

  • Flowcytometric classification is a standard diagnostic procedure for CVID.
  • Standardized guidelines are necessary for worldwide comparability of flowcytometric results in immunodeficiency centers.