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Updated: Jul 4, 2026

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
Coimmobilization of substrate and biocatalyst: A method for bioconversion of poorly soluble substances in water
R Kaul1, P Adlercreutz, B Mattiasson
1Department of Biotechnology, Chemical Center, University of Lund, P.O. Box 124, S-221-00 Lund, Sweden.
Abstract:
Coimmobilization of biocatalyst and substrate was studied as a method to increase the conversion rate in systems with substrates of extremely low solubility in water. The system studied was the conversion of hydrocortisone to prednisolone by Arthrobacter simplex. As a matrix for coimmobilization, alginate turned out to be superior to agar and agarose. After the reaction was complete, the beads were solubilized, andthe cells recovered for reuse, by centrifugation, whereas the prednisolone was extracted from the supernatant.
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