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Related Experiment Videos

Renin increases human amnion cell prostaglandin E2 biosynthesis.

S Lundin-Schiller1, M D Mitchell

  • 1Department of Obstetrics and Gynecology, University of Utah Medical Center, Salt Lake City 84132.

The Journal of Clinical Endocrinology and Metabolism
|August 1, 1991
PubMed
Summary
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Chorionic cells produce renin, an enzyme whose function was unclear. This study found that renin increases prostaglandin E2 (PGE2) production in amnion cells, a process independent of angiotensin. This suggests a new role for chorionic renin in pregnancy.

Area of Science:

  • Reproductive biology
  • Endocrinology
  • Biochemistry

Background:

  • Chorionic cells synthesize protein hormones, including prorenin/renin, with an undefined function.
  • The amnion, adjacent to the chorion, influences parturition via prostaglandin (PG) production.

Purpose of the Study:

  • To investigate the effect of renin on prostaglandin E2 (PGE2) biosynthesis in amnion cells.
  • To determine if renin's action on amnion cells is mediated by angiotensin.

Main Methods:

  • Primary monolayer cultures of amnion cells were incubated with varying concentrations of renin.
  • Prostaglandin E2 (PGE2) production was measured.
  • The effects of angiotensin-I (AI), angiotensin-II (AII), and renin with saralasin (an AII antagonist) were assessed.

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Main Results:

  • Renin induced a concentration-dependent increase in amnion cell PGE2 production.
  • Significant stimulation of PGE2 by renin was observed after 2 hours of incubation.
  • The stimulatory effect of renin was not inhibited by saralasin, and AI or AII alone did not stimulate PGE2 production.

Conclusions:

  • Chorionic renin stimulates amnion cell PGE2 production.
  • This effect appears to be independent of angiotensin formation.
  • Renin may have a novel regulatory role in amnion PGE2 production during pregnancy.