Related Experiment Video
Updated: Jul 4, 2026
Extraction of Lignin with High β-O-4 Content by Mild Ethanol Extraction and Its Effect on the Depolymerization Yield
Published on: January 7, 2019
Detailed analysis of modifications in lignin after treatment with cultures screened for lignin depolymerizing agents
Aarti Gidh1, Dinesh Talreja, Todd B Vinzant
1Department of Chemical Engineering, University of Mississippi, University, MS 38677, USA. agidh@olemiss.edu
Abstract:
Termites, beetles, and other arthropods can digest living and decaying wood plus other lignocellulosic plant litter. Microbial sources like other wood-eating insect guts and wastewater treatment sludge were screened for lignin depolymerization. Near infrared spectroscopy and atomic force microscopy (AFM) along with high-performance liquid chromatography (HPLC), were used to track changes in functional groups, size, shape, and molecular weight of lignin molecules during incubations. Odontotaenius disjunctus (Betsy beetle) guts dissected whole or separately as midgut, foregut, and hindgut, consumed corn stover but did not show lignin depolymerization. The sludge-treated lignin did show some reduction in molecular weight on the HPLC, particle size (350-650 nm initially to 135-220 nm by day 30) and particles per field on AFM. pH and the presence of nutrients had a substantial effect on the extent of depolymerization. Cultures in lignin and nutrients showed higher growth than cultures with lignin only. Colony characteristics within the beetle gut and the sludge were also evaluated.
More Related Videos
16:33Methods for Facilitating Microbial Growth on Pulp Mill Waste Streams and Characterization of the Biodegradation Potential of Cultured Microbes
Published on: December 12, 2013
11:31High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release
Published on: September 15, 2015