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Biochemical and morphological analysis of dentin following selective caries removal with a fluorescence-controlled
Søren Jepsen1, Yahya Açil, Thorsten Peschel
1Department of Operative Dentistry and Periodontology, University Hospital Bonn, 53111 Bonn, Germany. jepsen@uni-bonn.de
Background And Objective:
We evaluated the endpoint of caries removal with a novel fluorescence-controlled Er:YAG laser by biochemical analysis of dentin collagen and electron microscopy.
Materials And Methods:
For the biochemical analysis 210 carious and 60 caries-free teeth were treated by a fluorescence controlled Er:YAG laser (n=240) or by conventional drilling (n=30). A layer of dentin from the cavity floor was removed to determine collagen crosslinks hydroxylysylpyridinoline (HP) and lysylpyridinoline (LP) by HPLC. Thirty teeth were examined by SEM and TEM.
Results:
Approximately 100% HP and LP were found in carious dentin, in contrast to 0.33% (HP) and 0.68% (LP) in sound dentin. Following caries removal by laser 0.84% (HP) and 1.26% (LP) and 1.56% (HP) and 2.48% (LP) (threshold level 5, respectively 10) were found.
Conclusion:
The biochemical and ultrastructural analysis demonstrated the suitability of the fluorescence controlled Er:YAG laser to remove irreversibly denatured collagen of carious dentin.
