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Selective enrichment of cysteine-containing peptides using SPDP-functionalized superparamagnetic Fe(3)O(4)@SiO(2)
Arudra Palani1, Je-Sun Lee, Jiyoung Huh
1Department of Chemistry and Center for Electro- & Photo-Responsive Molecules, Korea University, Seoul, South Korea.
Journal of Proteome Research
|June 20, 2008
Abstract:
Superparamagnetic Fe(3)O(4)@SiO(2) core-shell nanoparticles (ca. 30 nm diameter), which are surface-modified with a thiol-specific functional group, exhibit superb capturing efficiency toward cysteinyl peptides without contamination from nonspecifically interacting peptides, as clearly evidenced through LC/MS/MS analysis.
