Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

Capillary Electrophoresis: Instrumentation

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

Electrophoresis: Overview

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

Two-dimensional Gel Electrophoresis

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...

Ion-Exchange Chromatography

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Low prevalence of SARS-CoV-2 in plasma of COVID-19 patients presenting to the emergency department.

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology·2020

Same author

A bioartificial kidney device with polarized secretion of immune modulators.

Journal of tissue engineering and regenerative medicine·2018

Same author

[A man with a curved deformity of the hand].

Nederlands tijdschrift voor geneeskunde·2017

Same author

Enantioselective capillary electrophoresis-mass spectrometry of amino acids in cerebrospinal fluid using a chiral derivatizing agent and volatile surfactant.

Analytica chimica acta·2016

Same author

Simultaneous assessment of protein heterogeneity and affinity by capillary electrophoresis-mass spectrometry.

Analytical chemistry·2015

Same author

Sensitive CE-MS analysis of potentially genotoxic alkylation compounds using derivatization and electrokinetic injection.

Analytica chimica acta·2015

Search research articles

Related Experiment Video

Updated: Jul 4, 2026

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

Recent developments in capillary isoelectric focusing.

L H H Silvertand¹, J Sastre Toraño, W P van Bennekom

¹Division of Biomedical Analysis, Department of Pharmaceutical Sciences, Faculty of Science, Utrecht University, P.O. Box 80082, 3508 TB Utrecht, The Netherlands. L.H.H.Silvertand@uu.nl

Journal of Chromatography. A

|June 21, 2008

Summary

This summary is machine-generated.

This review covers capillary isoelectric focusing (cIEF) technological advancements from 2003-2007, detailing new methods, detection techniques, and applications in biopharmaceutical analysis and metabolomics.

More Related Videos

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method

Published on: June 14, 2020

Related Experiment Videos

Last Updated: Jul 4, 2026

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

Published on: September 19, 2018

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method

Published on: June 14, 2020

Area of Science:

Analytical Chemistry
Separation Science
Biotechnology

Background:

Capillary isoelectric focusing (cIEF) is a powerful separation technique.
Continuous advancements are crucial for its application in complex analyses.
The period 2003-2007 saw significant technological developments in cIEF.

Purpose of the Study:

To review technological developments in capillary isoelectric focusing (cIEF) between 2003 and 2007.
To summarize key advancements in cIEF methodology, techniques, detection, and applications.
To highlight progress in miniaturization and multidimensional systems for complex sample analysis.

Main Methods:

Review of cIEF literature published between 2003-2007.
Focus on technological aspects including methodology, new techniques, detection, and applications.
Categorization of advancements into methodology, new systems, detection, multidimensional systems, miniaturization, and applications.

Main Results:

Methodology advancements include ampholyte composition, additives, carrier ampholyte-free cIEF, and capillary coatings.
New techniques encompass dynamic IEF and cIEF as an injection system.
Detection improvements involve whole column imaging, fluorescence, chemiluminescence, and mass spectrometry coupling.
Miniaturization efforts focused on microfluidic devices.
Applications highlighted include biopharmaceutical compound analysis and metabolomic studies using isolated proteins.

Conclusions:

Significant technological progress in cIEF occurred between 2003-2007.
Developments in methodology, detection, and miniaturization enhance cIEF's analytical capabilities.
Multidimensional systems are essential for analyzing complex biological matrices using cIEF.