Related Experiment Video
Updated: Jul 4, 2026
Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis
Published on: September 16, 2014
Detection of C-reactive protein on a functional poly(thiophene) self-assembled monolayer using surface plasmon
Hyun-Chul Kim1, Soo-Keun Lee, Won Bae Jeon
1Division of Nano-Bio Technology, Daegu Gyeongbuk Institute of Science and Technology, Daegu 704-230, Republic of Korea.
Abstract:
The preparation of a new poly(thiophene) with pendant N-hydroxysuccinimide ester groups and its application to immobilization of biomolecules are reported. A thiophene derivative of N-hydroxysuccinimide ester was polymerized with FeCl3 in chloroform and the resulting poly(thiophene) was characterized by nuclear magnetic resonance (NMR), Fourier transform infrared (FT-IR), and gel permeation chromatography (GPC). This polymer reacts with amine-bearing molecules to yield new poly(thiophene) derivatives and the specific interactions at the side groups could be detected. Thus, a self-assembled monolayer (SAM) using the polymer was formed on a gold-coated quartz cell and anti-C-reactive protein (anti-CRP) was immobilized. The binding behavior of CRP on the surface was monitored by use of a surface plasmon resonance (SPR) sensor system.
