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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

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Related Experiment Video

Updated: Jul 4, 2026

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

Lin-28 interaction with the Let-7 precursor loop mediates regulated microRNA processing.

Martin A Newman1, J Michael Thomson, Scott M Hammond

  • 1Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA.

RNA (New York, N.Y.)
|June 21, 2008
PubMed
Summary
This summary is machine-generated.

Embryonic cells use Lin-28 to block microRNA (miRNA) production. This protein inhibitor targets the Let-7 miRNA precursor, controlling its maturation during development.

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Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay
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Last Updated: Jul 4, 2026

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
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A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification
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Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification

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12:49

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

Published on: May 25, 2015

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • RNA Biology

Background:

  • MicroRNA (miRNA) expression is crucial for mammalian embryonic development.
  • Many miRNAs show unchanged transcription but reduced mature forms during development, indicating post-transcriptional regulation.

Purpose of the Study:

  • To elucidate the biochemical mechanism regulating the production of the Let-7 miRNA family.
  • To identify the factor responsible for inhibiting Let-7 processing in embryonic cells.

Main Methods:

  • Biochemical assays to study miRNA precursor processing.
  • RNA binding studies to identify inhibitor interaction sites.
  • Protein identification using biochemical and genetic approaches.

Main Results:

  • A Drosha Inhibitor in embryonic cells prevents the processing of Let-7 primary transcripts.
  • The inhibitor specifically binds to conserved nucleotides in the Let-7 precursor loop.
  • The embryonic stem cell-specific protein Lin-28 was identified as the Drosha Inhibitor.

Conclusions:

  • Lin-28 acts as a post-transcriptional regulator of Let-7 miRNA production.
  • This mechanism reveals a novel role for miRNA precursor loops in regulating mature miRNA output.
  • Findings establish a new microRNA post-transcriptional regulatory network involving Lin-28.