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Related Experiment Video

Updated: Jul 4, 2026

Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening
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Published on: January 16, 2021

A general method for phasing novel complex RNA crystal structures without heavy-atom derivatives.

Michael P Robertson1, William G Scott

  • 1Department of Chemistry and Biochemistry and The Center for Molecular Biology of RNA, University of California at Santa Cruz, Santa Cruz, CA 95064, USA.

Acta Crystallographica. Section D, Biological Crystallography
|June 21, 2008
PubMed
Summary
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Structural Simplicity and Mechanistic Complexity in the Hammerhead Ribozyme.

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Acta crystallographica. Section D, Biological crystallography·2014

Solving the crystallographic phase problem is crucial for determining macromolecular structures. A new method uses RNA structural fragments to solve complex ribozyme structures without modifications.

Area of Science:

  • Structural Biology
  • Biochemistry
  • Crystallography

Background:

  • The crystallographic phase problem hinders macromolecular structure determination.
  • Traditional methods often require heavy-atom derivatives or prior structural models.

Purpose of the Study:

  • To present a novel method for solving the crystallographic phase problem.
  • To determine the tertiary structure of a ribozyme without prior models or modifications.

Main Methods:

  • Utilizing idealized RNA secondary-structural fragments (A-form helices, GNRA tetraloops).
  • Employing an iterative molecular replacement procedure.
  • Applying solvent flattening to refine phase estimates.

Main Results:

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Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source
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Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella
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Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella

Published on: November 30, 2018

Related Experiment Videos

Last Updated: Jul 4, 2026

Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening
14:04

Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening

Published on: January 16, 2021

Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source
10:32

Sample Preparation and Transfer Protocol for In-Vacuum Long-Wavelength Crystallography on Beamline I23 at Diamond Light Source

Published on: April 23, 2021

Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella
11:31

Crystal Structure of the N-terminal Domain of Ryanodine Receptor from Plutella xylostella

Published on: November 30, 2018

  • Successfully solved the structure of a 142-nucleotide L1 ligase ribozyme heterodimer.
  • Obtained an interpretable electron-density map with minimized model bias.
  • Demonstrated success with other ribozymes, structured RNAs, and RNA-protein complexes.

    • Conclusions:

    • This fragment-based approach overcomes the crystallographic phase problem for RNA structures.
    • Enables structure determination without heavy-atom derivatives or existing models.
    • Broadly applicable to various RNA-containing molecular systems.