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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing02:01

Pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...

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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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Bioinformatics detection of alternative splicing.

Namshin Kim1, Christopher Lee

  • 1Molecular Biology Institute, Institute for Genomics and Proteomics, Department of Chemistry and Biochemistry, University of California, Los Angeles, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|June 21, 2008
PubMed
Summary

Genome-wide analysis of alternative splicing using Expressed Sequence Tag (EST) alignments reveals its regulatory roles. Databases built from this data enable querying for tissue-specific patterns and aid in experimental design.

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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Alternative splicing plays a crucial role in functional gene regulation.
  • Expressed Sequence Tag (EST) sequence alignments have become a powerful tool for genome-wide alternative splicing detection.
  • Understanding alternative splicing is key to deciphering complex biological processes.

Purpose of the Study:

  • To review the data, methodology, and challenges of genome-wide alternative splicing analyses.
  • To survey the applications of alternative splicing databases.
  • To highlight the potential of these databases for biological research and experimental design.

Main Methods:

  • Genome-wide detection of alternative splicing using EST sequence alignments with mRNA and genomic sequences.
  • Development of alternative splicing databases with schema design for querying specific patterns.
  • Estimation of exon inclusion/exclusion levels from EST alignments.

Main Results:

  • Genome-wide analyses have significantly expanded the understanding of alternative splicing's functional roles.
  • Alternative splicing databases allow querying for tissue-specific, disease-specific, gender-specific, or developmental stage-specific patterns.
  • EST alignments facilitate the estimation of exon splicing levels and inference of evolutionary changes.

Conclusions:

  • Alternative splicing databases are valuable resources for biological research.
  • These databases can automate the design of probes for high-throughput experimental validation of alternative splicing events.
  • The application of these databases aids in understanding gene regulation and disease mechanisms.