Multiplex pathogen detection based on spatially addressable microarrays of barcoded resins

Area of Science:

• Biotechnology
• Immunology
• Analytical Chemistry

Background:

• Suspension microsphere immunoassays offer versatile, high-throughput screening for antigen identification.
• Existing methods face challenges in complex biological fluid analysis.

Purpose of the Study:

• To develop a multiplex assay for pathogen antigen identification in complex biological fluids.
• To demonstrate the utility of antibody-functionalized barcoded resins (BCRs) for sensitive and specific biodetection.

Main Methods:

• Antibody-functionalized barcoded resins (BCRs) were employed for antigen capture.
• A dispersive Raman system with dual-laser excitation was used for simultaneous antibody and antigen identification.
• Tetraplex assays were performed to detect and quantify specific antigens.

Main Results:

• The BCR assay accurately identified and quantified anthrax protective antigen, Franciscella tularensis lipopolysaccharide, and CD14 antigens.
• A detection limit of 1 ng/mL was achieved for the tested antigens.
• The assay demonstrated high specificity and sensitivity in complex biological samples.

Conclusions:

• Antibody-functionalized barcoded resins provide a rapid, versatile, and simple platform for multiplex antigen detection.
• This technology shows significant potential for infectious disease biodetection and diagnostics.
• The reconfigurable microarray format enhances the adaptability of the BCR assay.