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Updated: Jul 4, 2026

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
Published on: April 14, 2016
Influence of particle size on bacterial community structure in aquatic sediments as revealed by 16S rRNA gene
Colin R Jackson1, Andrew Q Weeks
1Department of Biology, Shoemaker Hall, The University of Mississippi, University, MS 38677, USA. cjackson@olemiss.edu
Abstract:
Bacterial communities associated with sediment particles were examined using PCR-denaturing gradient gel electrophoresis and 16S rRNA gene sequencing. Particle size influenced community structure, with attached bacterial assemblages separating into 63- to 125-, 125- to 1,000-, and 1,000- to 2,000-microm fractions. Differences were particularly pronounced for the Verrucomicrobia-Planctomycetes, whose numbers were significantly reduced on coarser particles.
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