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Sample Preparation for Probe Electrospray Ionization Mass Spectrometry
Published on: February 19, 2020
Electrospray ionisation mass spectrometry: principles and clinical applications
C S Ho1, C W K Lam, M H M Chan
1Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong.
The Clinical Biochemist. Reviews
|June 24, 2008
Summary
Electrospray ionisation mass spectrometry (ESI-MS) is vital in clinical labs for metabolite analysis. This technique aids in diagnosing metabolic disorders and standardizing HbA1c measurements for better diabetes care.
Area of Science:
- Analytical Chemistry
- Clinical Chemistry
- Biochemistry
Background:
- Electrospray ionisation mass spectrometry (ESI-MS) is crucial for analyzing complex biological samples.
- Its role in clinical laboratories for structural and quantitative analysis is expanding.
Purpose of the Study:
- To provide a comprehensive overview of ESI-MS principles and clinical applications.
- To highlight ESI-MS advancements in disease diagnosis and biomolecule characterization.
Main Methods:
- Explanation of the electrospray ionisation process and mass spectrometer design.
- Discussion of mass spectrum characteristics and quantitative analysis considerations.
- Focus on ESI-tandem-MS for biomolecule analysis and structural elucidation.
Main Results:
- ESI-MS is effective for screening inborn errors of metabolism (amino acid, fatty acid, purine, pyrimidine).
- It aids in diagnosing galactosaemia and peroxisomal disorders.
- ESI-MS enables improved identification/quantification of haemoglobin variants and glycohaemoglobin standardization (HbA1c).
Conclusions:
- ESI-MS is a powerful tool for clinical diagnostics and biomolecule analysis.
- It offers significant advancements in standardizing diagnostic assays like HbA1c.
- ESI-MS will continue to shape clinical laboratory services, including therapeutic drug monitoring.
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