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Related Experiment Videos

Detection of circulating endothelial antigen.

C W Carson1, G G Hunder, K L Kaplan

  • 1Department of Medicine, Mayo Clinic/Foundation, Rochester, MN 55905.

The Journal of Rheumatology
|March 1, 1991
PubMed
Summary
This summary is machine-generated.

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Researchers developed an ELISA test to detect E92, an endothelial cell antigen, in patients with rheumatic diseases. Elevated E92 levels in patient serum suggest circulating endothelial antigens may indicate vascular involvement and endothelial cell function in rheumatic vascular syndromes.

Area of Science:

  • Immunology
  • Rheumatology
  • Vascular Biology

Background:

  • Rheumatic diseases can involve vascular complications.
  • Endothelial cell damage may occur in these conditions.
  • Biomarkers for endothelial cell function are needed.

Purpose of the Study:

  • To determine if endothelial cell antigens are detectable in serum from patients with rheumatic disease.
  • To investigate the presence of a specific endothelial cell antigen (E92) in rheumatic vascular syndromes.

Main Methods:

  • Developed an enzyme-linked immunosorbent assay (ELISA).
  • Utilized a monoclonal antibody against a 92,000 molecular weight endothelial cell specific antigen (E92).
  • Assayed serum samples from 191 patients with rheumatic disease and 34 healthy controls.

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Main Results:

  • E92 was undetectable or present in very low concentrations in healthy controls.
  • E92 was elevated in most patients with active rheumatic disease.
  • Circulating endothelial antigens were detected in patients with rheumatic vascular syndromes.

Conclusions:

  • Circulating endothelial antigens are present in rheumatic vascular syndromes.
  • E92 may serve as a potential biomarker for endothelial cell function in these conditions.
  • This finding could lead to improved diagnostics for rheumatic vascular involvement.