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Updated: Jul 4, 2026

Inducing Apical Periodontitis in Mice
10:26

Inducing Apical Periodontitis in Mice

Published on: August 6, 2019

Microbial distribution in the root canal system after periapical lesion induction using different methods.

Juliane Maria Guerreiro Tanomaru1, Mario Roberto Leonardo, Mário Tanomaru-Filho

  • 1Department of Restorative Dentistry, Dental School of Araraquara, São Paulo State University, Araraquara, SP, Brazil.

Brazilian Dental Journal
|June 24, 2008
PubMed
Summary

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This study examined microbial distribution in dog root canals after inducing periapical lesions. Microorganisms were found throughout the root canal system, particularly on walls and in dentinal tubules, regardless of lesion induction method.

Area of Science:

  • Microbiology
  • Endodontics
  • Veterinary Dentistry

Background:

  • Periapical lesions are a common complication of endodontic infections.
  • Understanding microbial distribution is crucial for effective treatment.
  • Various methods exist for inducing experimental periapical lesions.

Purpose of the Study:

  • To evaluate microbial distribution within the root canal system.
  • To compare microbial presence after different periapical lesion induction methods.
  • To identify specific sites of microbial colonization in canine teeth.

Main Methods:

  • Induction of periapical lesions in 52 dog root canals using varied oral exposure and apex perforation.
  • Microbial assessment using the Brown & Brenn staining method.

Related Experiment Videos

Last Updated: Jul 4, 2026

Inducing Apical Periodontitis in Mice
10:26

Inducing Apical Periodontitis in Mice

Published on: August 6, 2019

  • Statistical analysis of microbial incidence using the chi-square test.

    • Main Results:

    • Microorganisms were detected throughout the entire root canal system in all experimental groups.
    • Higher microbial prevalence was observed on root canal walls, apical delta, and dentinal tubules (p<0.05).
    • Cementum and cemental resorption areas also showed microbial presence.

    Conclusions:

    • Different methods for inducing periapical periodontitis resulted in similar microbial distribution patterns.
    • The root canal walls, apical delta, and dentinal tubules are primary sites for microbial colonization.
    • Effective disinfection strategies must target these specific areas within the root canal system.