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Updated: Jul 4, 2026
In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
Published on: November 28, 2019
Oxytocin receptor- and Oct-4-expressing cells in human amniotic fluid
Konstantinos Stefanidis1, Dimitris Loutradis, Vasiliki Anastasiadou
1Laboratory of Stem Cells, Division of Reproductive Medicine, Alexandra Maternity Hospital, Athens, Greece. konstef@hol.gr
Background/Aims:
The present clinical and molecular study aimed at investigating the presence of the genes encoding oxytocin receptor (OT-R) and Oct-4 in human amniotic fluid cells.
Methods:
Amniotic fluid samples were obtained from amniocentesis. Cells from human amniotic fluid samples were analyzed for mRNA expression of OT-R and Oct-4 via reverse transcription-polymerase chain reaction (RT-PCR). Immunocytochemistry was also performed with OT-R and Oct-4 antibodies.
Results:
RT-PCR from 10 independent amniocentesis samples demonstrated the expression of OT-R and Oct-4 mRNA. The cells also showed strong immunoreactivity for molecular markers of OT-R and Oct-4.
Conclusion:
OT-R and Oct-4 are expressed in human amniotic fluid cells. The role of oxytocin in the physiology and pathophysiology of amniotic fluid cells remains to be settled.
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