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Published on: March 24, 2015
Age-dependent decrease in serum soluble interferon-gamma receptor (sIFN-gammaR) in healthy Japanese individuals;
Yoshihiro Okamoto1, Kyoko Seta, Izumi Nakajo
1Laboratory of Immunology and Microbiology, Faculty of Pharmacy, Chiba Institute of Science, Choshi, Chiba, Japan. yokamoto@cis.ac.jp
Insights
Serum soluble interferon-gamma receptor (sIFN-gammaR) levels are higher in healthy children than adults. Age-matched controls are crucial for interpreting pediatric sIFN-gammaR levels in clinical diagnostics.
Area of Science:
- Immunology
- Pediatrics
- Clinical Chemistry
Background:
- The clinical utility of serum soluble interferon-gamma receptor (sIFN-gammaR) requires established reference values.
- Pediatric reference ranges for sIFN-gammaR are currently unavailable, hindering diagnostic applications.
Purpose of the Study:
- To determine the normal serum sIFN-gammaR levels in healthy Japanese children.
- To establish age-specific reference values for pediatric sIFN-gammaR.
- To assess the preliminary diagnostic utility of sIFN-gammaR in pediatric hemolytic uremic syndrome (HUS).
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA) was employed to measure serum sIFN-gammaR levels.
- Serum samples were collected from healthy Japanese children (0-14 years) and adults (>15 years).
- Statistical analysis was performed to compare sIFN-gammaR levels between age groups.
Main Results:
- Serum sIFN-gammaR levels were significantly higher in children compared to adults (p < 0.01).
- A total of 104 healthy individuals (children and adults) were analyzed.
- Preliminary data suggests potential application of sIFN-gammaR as a diagnostic marker in diarrhea-positive HUS.
Conclusions:
- Age-matched controls are essential for accurate interpretation of serum sIFN-gammaR levels in pediatric patients.
- Establishing pediatric reference ranges for sIFN-gammaR is critical for its clinical application.
- Further research is warranted to validate sIFN-gammaR as a diagnostic tool for pediatric HUS.
Abstract:
We planned to investigate the clinical significance of serum soluble interferon-gamma receptor (sIFN-gammaR) level in pediatric patients. The diagnostic application of the measurement of serum sIFN-gammaR level depends critically on the control value. However, there is no information of the control value of serum sIFN-gammaR for children. In the present study, we determined the serum sIFN-gammaR level of healthy Japanese children using an ELISA. The serum sIFN-gammaR level of children (0-14 years old) was significantly higher than that of adults (over 15 years old) (p < 0.01, n = 104). Thus, it is recommended that, when the serum sIFN-gammaR level of patients is evaluated, it should be compared against age-matched controls. We also preliminarily applied this assay as a diagnostic parameter for the patients with diarrhea positive (D+) hemolytic uremic syndrome (HUS).
