Related Experiment Video
Updated: Jul 4, 2026
Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice
Published on: December 4, 2020
Miniaturization of primary porcine urinary bladder epithelial cell cultures and application for gene expression
Michael Alexander1, Claudia Behm, Wolfram Follmann
1Department of Structural Biology, Max Planck Institute for Molecular Physiology, Dortmund, Germany.
Abstract:
Benzo[a]pyrene (BaP) is an environmental pollutant used as a key marker substance for polycyclic aromatic hydrocarbons (PAHs). PAHs are believed to play a prominent role in the development of bladder cancer. A test system based on primary porcine urinary bladder epithelial cells (PUBEC) has been utilized as an in vitro model for urinary bladder epithelium. Recently in PUBEC cultures derived from pools of several bladders potent induction of CYP1A1 was detected after BaP treatment. Results from a modified approach using miniaturized PUBEC cultures for the analysis of individual bladder specimens with regard to cell growth and to BaP-mediated induction of CYP1A1 mRNA expression are presented herein. Two types of responses, low and high CYP1A1 induction among individual bladder specimens from eight donor animals, were detected. All of these tissue samples expressed the wild-type genotype of CYP1A1.
