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Updated: Jul 4, 2026
A Simple Fluorescence-based Reporter Assay to Identify Cellular Components Required for Ricin Toxin A Chain (RTA) Trafficking in Yeast
Published on: December 15, 2017
Biochemical properties of ricin in immature castor seed
Srinivas V S Chakravartula1, Nagaraj Guttarla
1Natural Products and Medicinal Chemistry Laboratory, Island Vitamins - Venus Pharmaceuticals Inc., Farmingdale, New York 11735, USA. srinivas@venuspharmaceuticals.com
Abstract:
The biochemical properties of ricin at different stages of seed i.e. from immature to mature seed were studied. Hemagglutination, SDS-PAGE and UV-spectrometry studies showed total absence of RCA protein in the immature seed. Interestingly, ricin extract on SDS-PAGE showed only one protein band with a molecular weight of 29,000 dalton corresponding to the molecular weight of A chain of ricin. Our results have shown that at immature seed level only the toxic moiety of ricin (A chain) is being synthesized first and gradually the RCA and B chain of ricin.
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