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Microfluidic Platform with Multiplexed Electronic Detection for Spatial Tracking of Particles
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Multiplexing ligand-receptor binding measurements by chemically patterning microfluidic channels.

Jinjun Shi1, Tinglu Yang, Paul S Cremer

  • 1Department of Chemistry, Texas A&M University, College Station, Texas 77843, USA.

Analytical Chemistry
|June 24, 2008
PubMed
Summary

This study introduces a novel method for patterning supported phospholipid bilayers (SLBs) using deep UV lithography and bovine serum albumin (BSA). This technique enables precise control over lipid bilayer arrays for studying molecular interactions, like ganglioside GM1 binding with cholera toxin B (CTB).

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Area of Science:

  • Biophysics
  • Materials Science
  • Microfluidics

Background:

  • Supported lipid bilayers (SLBs) are crucial for biomimetic studies.
  • Patterning SLBs with high spatial resolution is challenging.
  • Microfluidic devices offer controlled environments for biological assays.

Purpose of the Study:

  • To develop a versatile method for patterning SLBs on surfaces and in microfluidic channels.
  • To create spatially defined arrays of lipid bilayers with varying compositions.
  • To investigate the binding interactions between ganglioside GM1 and cholera toxin B (CTB) subunits.

Main Methods:

  • Formation of bovine serum albumin (BSA) monolayers at the liquid/solid interface.
  • Selective patterning of BSA monolayers using deep UV lithography.

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  • Deposition of SLBs via vesicle fusion onto patterned BSA areas.
  • Fabrication of a seven-channel microfluidic device for simultaneous analysis.
  • Utilizing total internal reflection fluorescence microscopy for binding studies.

    • Main Results:

    • Spatially addressed bilayer arrays were successfully formed with BSA acting as corrals.
    • Microfluidic channels were patterned with SLBs containing distinct concentrations of ganglioside GM1 (0-2.0 mol %).
    • Equilibrium dissociation constants for CTB-GM1 interactions were simultaneously determined at various ligand densities.

    Conclusions:

    • The developed method allows for precise patterning of SLBs for creating complex lipid architectures.
    • This approach facilitates high-throughput analysis of molecular binding events in microfluidic systems.
    • The study provides quantitative insights into the CTB-GM1 binding dynamics as a function of GM1 density.