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[Analysis of the kinematic parameters of hyperactivated Guinea pig spermatozoa]
Lu Xu1, Jing-Yan Huang, Qun-Wei Cui
1College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China. xulu829@126.com
Objective:
To evaluate the role of sperm kinematic parameters in the hyperactivation of Guinea pig spermatozoa, and to confirm the index of their hyperactivated motility.
Methods:
Computer-aided sperm analysis (CASA) was used to describe the kinesis parameters of the Guinea pig spermatozoa incubated for 1, 3, 5 and 7 hours.
Results:
The curvilinear velocity, average path velocity and amplitude of lateral head displacement were increased with time and reached the peak at 5 hours, while the straight linear velocity, linearity, straightness and beat cross frequency were gradually decreased with time and hit the bottom at 5 hours.
Conclusion:
The sperm movement pattern changes greatly before hyperactivation during the capacitation of Guinea pig spermatozoa.
