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Updated: Jul 4, 2026

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis
Published on: June 24, 2018
Distinct actions of strontium on mineral formation in matrix vesicles
Géraldine Bechkoff1, Jacqueline Radisson, Laurence Bessueille
1Université de Lyon, Villeurbanne F-69622, France.
Abstract:
Matrix vesicles (MVs) are involved in the initial step of mineralization in skeletal tissues and provide an easily model to analyze the hydroxyapatite (HA) formation. Sr stimulates bone formation and its effect was tested on MVs. Sr(2+) (15-50 microM) in the mineralization medium containing MVs, 2 mM Ca(2+) and 3.42 mM P(i), retarded HA formation. Sr(2+) (1-5 mM) in the same medium-induced other types of mineral than HA and cancelled the ATP-, ADP- or PP(i)-induced retardation in the mineral formation. Our findings suggest that the beneficial effect of Sr(2+) at a low dose (15-50 microM) is rather an inhibitor of bone resorption than an activator of mineral formation, while at high Sr(2+) concentration (1-5 mM), mineral formation, especially other types of mineral than HA, is favored.
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