Related Experiment Video
Updated: Jul 4, 2026
Analyzing Platelet Subpopulations by Multi-color Flow Cytometry
Published on: June 10, 2025
Quality analysis of red cell and platelet concentrates obtained by the automated 'Top-and-Top' blood processing
Sudipta Sekhar Das1, Shamee Shastry, Rajendra Chaudhary
1Department of Transfusion Medicine, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Raebareily Road, Lucknow, India.
The efficacy of the widely practiced 'Top and Bottom' collection system has been established. We studied efficacy of our new 'Top-and-Top' automated blood component separation system with regards to quality analysis of red cell and platelet concentrates. At installation of machine 59 U of whole blood (WB) were used for its calibration and validation program. Optimum volume, leukocyte-platelet recovery and red cells loss in BC were adjusted as per recommended standards. WB showed mean volume of 510 ml and net hemoglobin content of 63 g/bag. BC recovered 91.7% and 62.7% of platelet and leukocytes, respectively, in a mean volume of 96 ml a hematocrit of 54% and mean platelet of 5.47 x 10(10) were observed in red cell and platelet concentrates, respectively. In many aspects quality of our products could not comply with the recommended European and American standards and this requires a close insight into the series of activities associated with WB collection, separation and quality control program.
The efficacy of the widely practiced 'Top and Bottom' collection system has been established. We studied efficacy of our new 'Top-and-Top' automated blood component separation system with regards to quality analysis of red cell and platelet concentrates. At installation of machine 59 U of whole blood (WB) were used for its calibration and validation program. Optimum volume, leukocyte-platelet recovery and red cells loss in BC were adjusted as per recommended standards. WB showed mean volume of 510 ml and net hemoglobin content of 63 g/bag. BC recovered 91.7% and 62.7% of platelet and leukocytes, respectively, in a mean volume of 96 ml a hematocrit of 54% and mean platelet of 5.47 x 10(10) were observed in red cell and platelet concentrates, respectively. In many aspects quality of our products could not comply with the recommended European and American standards and this requires a close insight into the series of activities associated with WB collection, separation and quality control program.
More Related Videos
10:25Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion In Vitro
Published on: March 19, 2016
09:38A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
Published on: February 14, 2017