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[Detection of virulence markers in clinical strains of Yersinia]
Abstract:
The dispersion of plasmid pYV associated virulence markers in 474 Yersinia strains isolated from people has been studied. The ability to autoagglutination, calcium dependence of growth and the specific antigens were identified in 157 strains of traditionally pathogenic Yersinia enterocolitica serovars 03, 09, Yersinia pseudotuberculosis serovar I. They were not found in 223 strains of other 12 serovars of Yersinia enterocolitica, in 40 strains of Yersinia frederiksenii, Yersinia kristensenii, Yersinia intermedia. The proportion of virulent clones in the population of Yersinia is noted to depend on the conditions of its existence in vivo or in vitro. Identification of virulence markers is acknowledged to be expedient in epidemiological and ethiological estimation of the role of isolated Yersinia strains.
Insights
Virulence markers on plasmid pYV were studied in Yersinia strains. Pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis strains showed these markers, unlike other Yersinia species.
Area of Science:
- Microbiology
- Genetics
- Pathogen research
Context:
- Yersinia strains are significant human pathogens.
- Plasmid pYV carries crucial virulence factors.
- Understanding virulence marker dispersion is key to controlling infections.
Purpose:
- To investigate the distribution of plasmid pYV-associated virulence markers in human-isolated Yersinia strains.
- To differentiate pathogenic Yersinia strains based on virulence marker presence.
Summary:
- 474 Yersinia strains were analyzed for virulence markers.
- Markers like autoagglutination and calcium dependence were found in pathogenic Yersinia enterocolitica (serovars 03, 09) and Yersinia pseudotuberculosis (serovar I).
- These markers were absent in other Yersinia enterocolitica serovars and related species (Y. frederiksenii, Y. kristensenii, Y. intermedia).
Impact:
- Identifies specific virulence markers for pathogenic Yersinia strains.
- Highlights the dependence of virulent clone proportion on environmental conditions (in vivo vs. in vitro).
- Supports the expediency of virulence marker identification for epidemiological and etiological assessments of Yersinia.