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Updated: Jul 4, 2026
Intravital Microscopy of Leukocyte-endothelial and Platelet-leukocyte Interactions in Mesenterial Veins in Mice
Published on: August 13, 2015
P-selectin-dependent monocyte recruitment through platelet interaction in intestinal microvessels of LPS-treated mice
Masaaki Higashiyama1, Ryota Hokari, Hisayuki Matsunaga
1Department of Internal Medicine, National Defense Medical College, Saitama, Japan.
Background:
Although platelets or monocytes are thought to be involved in intestinal inflammation, there has been no report on whether platelets can modulate monocyte recruitment in intestinal microvessels. The objective of this study was to determine whether blockade of platelet adhesion attenuates monocyte recruitment in inflamed murine intestinal microvessels.
Methods:
Monocytes and platelet-rich plasma were obtained from C57B6/J mice. Interaction of monocytes and platelets with intestinal microvessels was observed under an intravital microscope. Lipopolysaccharide (LPS) was administered intraperitoneally. The effects of anti-P-selectin or anti-platelets antibody treatments or phosphodiesterase (PDE) inhibitors (PDE-3 and PDE-2/4 inhibitor) treatments were also studied.
Results:
LPS-treatment increased the rolling and adhesion of both platelets and monocytes. Pretreatment with an anti-P-selectin antibody inhibited the increased platelet adhesion to venular walls and also attenuated the monocyte adhesion. A PDE-2/4 inhibitor (ibuzilast) also ameliorated both platelet and monocyte adhesion. A PDE-3 inhibitor (cilostazol) ameliorated only monocyte adhesion without directly affecting the adhesion of platelets to microvessels.
Conclusions:
We observed inhibition of platelets adhesion attenuated monocytes recruitment in intestinal microvessels. Attenuation of LPS induced monocyte adhesion by a specific PDE-3 inhibitor suggests that P-selectin on activated platelets may play an important role through monocyte and platelet interaction.
Insights
Blocking platelet adhesion reduces monocyte recruitment in the intestines. This suggests that platelet-P-selectin interactions are key in intestinal inflammation, offering potential therapeutic targets.
Area of Science:
- Immunology
- Gastroenterology
- Hematology
Background:
- Platelets and monocytes are implicated in intestinal inflammation.
- The role of platelet adhesion in modulating monocyte recruitment in intestinal microvessels remains unclear.
Purpose of the Study:
- To investigate if blocking platelet adhesion can reduce monocyte recruitment in inflamed murine intestinal microvessels.
Main Methods:
- Intravital microscopy was used to observe monocyte and platelet interactions in mouse intestinal microvessels.
- Lipopolysaccharide (LPS) induced inflammation.
- The effects of anti-P-selectin antibodies and phosphodiesterase (PDE) inhibitors were evaluated.
Main Results:
- LPS increased platelet and monocyte adhesion.
- Anti-P-selectin antibodies and a PDE-2/4 inhibitor (ibuzilast) reduced both platelet and monocyte adhesion.
- A PDE-3 inhibitor (cilostazol) reduced monocyte adhesion but not platelet adhesion.
Conclusions:
- Inhibiting platelet adhesion effectively reduces monocyte recruitment in intestinal microvessels.
- P-selectin on activated platelets appears crucial for monocyte-platelet interactions in LPS-induced intestinal inflammation.
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