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Related Experiment Video

Updated: Jul 4, 2026

Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing

Multimodal Quantitative Phase Imaging with Digital Holographic Microscopy Accurately Assesses Intestinal Inflammation and Epithelial Wound Healing

Published on: September 13, 2016

Quantitative phase imaging by three-wavelength digital holography.

Christopher J Mann¹, Philip R Bingham, Vincent C Paquit

¹Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA. manncj@ornl.gov

|June 26, 2008

Summary

This summary is machine-generated.

Three-wavelength digital holography achieves high-precision surface height measurements over several microns. This method preserves single-wavelength precision using synthetic wavelengths and hierarchical phase unwrapping for real-time applications.

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Last Updated: Jul 4, 2026

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Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

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Published on: February 8, 2014

Area of Science:

Optics and Photonics
Metrology

Background:

Digital holography offers precise surface measurement capabilities.
Single-wavelength holography is limited in measurement range.
Multi-wavelength approaches can extend range but may compromise precision.

Purpose of the Study:

To develop a three-wavelength digital holography technique for extended range surface height measurement.
To maintain high precision comparable to single-wavelength methods.
To enable real-time measurement performance.

Main Methods:

Application of three-wavelength digital holography.
Generation of intermediate synthetic wavelengths from the three input wavelengths.
Utilizing hierarchical optical phase unwrapping algorithms.
Simultaneous capture of complex wavefronts for each wavelength in a single digital image.

Main Results:

Achieved surface height measurements over several microns range.
Maintained low noise precision characteristic of single-wavelength phase measurement.
Demonstrated the feasibility of real-time performance due to simultaneous wavefront capture.

Conclusions:

Three-wavelength digital holography effectively extends measurement range while preserving precision.
The combination of synthetic wavelengths and hierarchical phase unwrapping is key to the technique's success.
This method is suitable for real-time, high-precision surface metrology applications.