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Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses
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Published on: November 16, 2016

Secretion of human interferon-beta 1b by recombinant Lactococcus lactis

Zhong Zhuang1, Zhi-Gang Wu, Min Chen

  • 1State Key Laboratory of Microbial Technology, Shandong University, Jinan, Shandong 250100, People's Republic of China.

Biotechnology Letters
|June 26, 2008
PubMed

Interferon-beta has anti-viral, anti-proliferation and multifunctional immunomodulatory activities and shows promising clinical effects for treatment of inflammatory disorders. The recombinant human interferon-beta (huIFN-beta) 1b was expressed in the food-grade lactic acid bacterium, Lactococcus lactis, using a nisin-controlled gene expression system. huIFN-beta production from recombinant strains (with and without LEISSTCDA propeptide) was approximately 21 and 7 microg l(-1), respectively. Moreover, 95% (former strain) and 88% (latter strain) of total recombinant proteins were secreted into the culture medium. The biological activities of huIFN-beta from recombinant strains revealed similar antiviral activities of 10(7) I.U. mg(-1). These results demonstrate the potential application of recombinant strains as a food grade vehicle to deliver bioactive huIFN-beta in vivo.

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