Downstream processing of inulinase. Comparison of different techniques

Candida kefyr DSM 70106 was cultivated in a medium containing inulin as a carbon source. About 92% of the inulinase was recovered directly from the medium. Different concentration (C(f)) and enrichment (E(f)) factors were obtained, using the following methods: Cross-flow filtration (microfiltration and cell diafiltration were carried out using a rotary filter; enzyme ultrafiltration and diafiltration were performed using a cassette module): C(f) = 7.5 and E(f) = 2.2; liquid-liquid extraction of N-Benzyl-N-Dodecyl-N-bis[2-hydroxyethyl] ammonium chloride (BDBAC) reversed micelles: C(f) = 2.5 and E(f) = 2.7; and expanded-bed adsorption: C(f) = 2.8 and E(f) = 4.3.